Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD4

Cell type

Cell type Class
Lung
Cell type
NCI-H209
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Small Cell

Attributes by original data submitter

Sample

source_name
SCLC cell line
cell line
NCI-H209
genotype/variation
expressing 5SA mutant human YAP
sample type
TEAD4 ChIP
chip antibody
TEAD4 (Santa Cruz sc-101184)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in formaldehyde, lysed and sonicated to fragment the gDNA. Clarified gDNA was immunoprecipitated with an anti-TEAD4 antibody, beads were washed then eluted and crosslinks reversed. Libraries were prepared using the Illumina TruSeq ChIP Sample Prep Kit (cat#: IP-202-101) according to the manufacturer's protocol with 18 cycles for final PCR enrichment. Samples were size selected using PippinHT (Sage Science) with 2% gel, targeting 300 bp fragments (start at 239 bp, end at 361 bp, and tight on Range Flag).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
44209995
Reads aligned (%)
98.2
Duplicates removed (%)
20.0
Number of peaks
13103 (qval < 1E-05)

hg19

Number of total reads
44209995
Reads aligned (%)
97.2
Duplicates removed (%)
21.3
Number of peaks
12805 (qval < 1E-05)

Base call quality data from DBCLS SRA