Antigen

Antigen Class

TFs and others

Antigen

TEAD4

Cell type

Cell type Class

Lung

Cell type

NCI-H209

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma Small Cell

Sample

source_name

SCLC cell line

cell line

NCI-H209

genotype/variation

expressing 5SA mutant human YAP

sample type

TEAD4 ChIP

chip antibody

TEAD4 (Santa Cruz sc-101184)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked in formaldehyde, lysed and sonicated to fragment the gDNA. Clarified gDNA was immunoprecipitated with an anti-TEAD4 antibody, beads were washed then eluted and crosslinks reversed. Libraries were prepared using the Illumina TruSeq ChIP Sample Prep Kit (cat#: IP-202-101) according to the manufacturer's protocol with 18 cycles for final PCR enrichment. Samples were size selected using PippinHT (Sage Science) with 2% gel, targeting 300 bp fragments (start at 239 bp, end at 361 bp, and tight on Range Flag).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

44209995

Reads aligned (%)

98.2

Duplicates removed (%)

20.0

Number of peaks

13103 (qval < 1E-05)

hg19

Number of total reads

44209995

Reads aligned (%)

97.2

Duplicates removed (%)

21.3

Number of peaks

12805 (qval < 1E-05)